Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet: Preparation of the 16S rRNA gene qRT-PCR to assess bacterial load

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques: SYBR Green Assay

Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet: The PCR cycling conditions for each 16S rRNA gene qRT-PCR assay

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques:

Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet: Antibiotic treatment (ABX) results in significantly reduced bacterial load in antibiotics exposed dams Bacterial load as determined by copies of the 16S rRNA genes in fecal samples collected from antibiotic treated (ABX) and untreated dams (NO ABX) at day 21 post-birth. Data are represented as mean ± SEM. A Mann Whitney test was used to assess statistical significance, where ∗∗∗p < 0.001.

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques: MANN-WHITNEY

Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet: Antibiotic treatment (ABX) results in significantly reduced bacterial in antibiotics pups Bacterial load as determined by copies of the 16S rRNA genes in fecal samples collected from antibiotic treated (ABX) and untreated dams (NO ABX) at day 21 post-birth. Data are represented as mean ± SEM. A Mann Whitney test utilized to assess statistical significance, where ∗∗∗∗p < 0.0001.

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques: MANN-WHITNEY

Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet: Composition of the fecal microbiota in antibiotic exposed and unexposed mice throughout life The composition of microbiota in unexposed mice (no ABX, n=20) and mice exposed to antibiotics in early life (n=20) was profiled using 16S rRNA gene sequencing of DNA extracted from pooled fecal samples collected at specific intervals from 1 week post-antibiotic exposure (week 4 of age) to week 102 of life. Two distinct microbiota community types were evident: post-antibiotic exposure (PAM I and PAM II, n=10/group). Note: Reprinted from “The composition of the gut microbiota following early life antibiotic exposure affects host health and longevity in later life,” by ( Lynn et al., 2021 ). Reprinted with permission.

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques: Sequencing

Journal: STAR Protocols

Article Title: Protocol to assess the impact of early-life antibiotic exposure on murine longevity

doi: 10.1016/j.xpro.2022.101220

Figure Lengend Snippet:

Article Snippet: For an in-depth protocol for 16S rRNA gene sequencing library preparation, please refer to the Nextera XT Index kit.

Techniques: Recombinant, Virus, SYBR Green Assay, Sequencing, Software, Real-time Polymerase Chain Reaction